Rohr had released a 30-man provisional squad for the 2018 FIFA World Cup with 29 of them reporting for the camp which opened on Tuesday, May 22.
After the friendly game against DR Congo on Monday, May 28 which ended 1-1, Rohr has dropped four players from the squad.
Plateau United goalkeeper Dele Ajiboye, defender Stephen Eze, striker Junior Lokosaand midfielder Uche Agbo are the players that have been dropped.
Lokosa made his Super Eagles debut in a 71-minute appearance as Nigeria drew DR Congo 1-1 on Monday.
Ajiboye, Eze and Agbo were all on the bench for the game.
Moses Simon who is struggling with an injury is surprisingly not among the players that have been dropped.
The forward will follow the team to London where he will have another scan.
He is expected to be among the three other players that will be dropped before the 2018 FIFA World Cup in Russia.